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Preanalytická fáze laboratorního vyšetření
  Martin Beránek
  ÚKBD = FN HK
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 přednáška
Bioanalytika
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Tři fáze laboratorního vyšetření=
Preanalytická fáze
Analytická fáze
Postanalytická fáze
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text/html; charset="us-ascii" Preanalytická fáze laboratorního vyšetření
Čím preanalytická fáze ovlivňuje výsledek:
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Tok nových informací o preanalytických vlivech
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ElAF4uJeglosyCAW/j24RYQSQkhhhewccB+GX0jH4YcghijiEx6OCIWDJv6QWYpQ/MbiiRe+KFCM MuZQVo0n4qjjjjzquGGPPtAI5AtCDskCikauYFKSOKzIZJNFclNiT1PGk9KPT9YQZZYmuMilC0Ax cI4+XxqIwJloqqePPlgKmA455mC5pppzHiDmaHVuCdVuui0pAQN5dlanoGiieU5Hfs4EZzmMyjln SINe9Whnh5Ij5p1EVglTn55dwKmTEzBQ6D7ppIIkSwfFGRIAl7K6jXllKhkrLhEAADs= ------=_NextPart_01C87D67.A5C984D0 Content-Location: file:///C:/1FCBB1C1/preanalytika_soubory/slide0004.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Preanalytická fáze laboratorního vyšetření
Pacient – zdroj variability
=
<= span style=3D'font-family:Arial;color:yellow'>•Pohlaví (♂: ↑ GGT, CK, UREA, Fe, 1= 7-OH steroidy)
•Etnikum  černoši: ↑ TP, IgG o 40%, CK, LD,
•        = ;            &n= bsp;            = ;          ↓ CHOl, TAG, Hb, leu
•        = ;          asiaté: ↑ AMYL, KM (N. Zéland)
<= span style=3D'font-family:Arial;color:yellow'>•Věk (děti: <= span lang=3DCS style=3D'font-family:Arial;mso-ascii-font-family:Arial;mso-bidi-= font-family: Arial;mso-hansi-font-family:Arial;font-size:75%;color:white;mso-color-inde= x: 0'>↑ <= span lang=3DCS style=3D'font-family:Arial;mso-ascii-font-family:Arial;mso-hansi= -font-family: Arial;font-size:75%;color:white;mso-color-index:0'>ALP, ACP, P, ↓ KREA, TAG, UREA)
•Biologické cykly (hormony, KREA, Fe)
<= span style=3D'font-family:Arial;color:yellow'>•Gravidita (↑ hCG, α1FP, CHOL, ↓ Hb, KREA, UREA<= /span>)
<= span style=3D'font-family:Arial;color:yellow'>•Současné choroby (jaterní choroby vs IM<= span lang=3DCS style=3D'font-family:Arial;mso-ascii-font-family:Arial;mso-hansi= -font-family: Arial;color:white;mso-color-index:0'>)
<= span style=3D'font-family:Arial;color:yellow'>•Biologický poločas analytu (Alb vs pre-alb)
•<= /span>
Faktory neovlivnitelné preanalyticky
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Pacient
faktory=         = ;             preanalyticky  <= /div>
•Fyzická aktivita (hemokonc. - ↑= ; Alb, ↑= ; CK, AST, LD, MYO, ↓= ; pH, ↑= ; laktát, centralizace obě= hu – ↑ KREA, UREA, ↓= ; TAG, GLU, MK, ↑= ; ketony, ↔ hormony)
•Psychický stres (↑ nadledv. hormony, GLU, NEMK)
•Strava (= ↔ GLU, TAG, NEMK, UREA, KM, P, pH moči)
•Tekutiny (hemokonc., avitaminosy, alkohol = 211; ↑ <= /span>TAG, AST, ALT, GGT, ↓= ; GLU, ↑= ; KM)
•Kouření (↑ cHb, fibrinogen, ALP, Hb, CEA, ery)&#= 13;
•Léky (↔ metabolismus, interference, parent. výživa)
•Operační výkon (léky, ↑= ; CK, AST, LD, Myo, hormony)
•
ovlivnitelné
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Základ= ní informace pro pacienta před odběrem   
•odběr mezi 6-8 hodinou ranní
•24-48H bez intenzivní fyzické aktivity
•den před odběrem vynechat léky, které nejsou nezbytné
•10-12H před odběrem lačnit, ale doplňovat tekutiny !
•ráno bez větš&iac= ute; fyzické aktivity (běh, kolo)
•před odběrem nepít alkohol ani kávu
•15 min před odběrem v kl= idu sedět
•u speciálních testů dodržovat předepsanou přípravu
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Typy biologického materiá= lu v KBch
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>Krev (venózní, kapilární, arteriální)
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>Plasma
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>Sérum
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>Moč
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>Stolice
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>Pot <= /div>
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>Tekutiny: synoviální, likvor, amniová, pleurální,
•        = ;            perikardiální, z břišní dutiny <= /span>
•Vzorek pevné tkáně (kostní dřeň, nádor, biopsie, …) <= /span>
<= span style=3D'font-size:88%;color:white;mso-color-index:0;visibility:hidden'>•<= /span>
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Odběr biologického materiálu
•Identifikace zkumavky =3D identifikace žádanky
•Primární odběrové soupravy eliminují lidskou chybu a kontaminaci při manipulaci se vzorkem
•Čas srážení (30 min) pro přípravu séra !
•Odběrový plast (PP, PE) – kaolin ↓ čas srážení&= #13;
•Plast – propustný pro plyny !!
     (=3D> na vzduchu ↑ pO2, ↓ pCO2)
•= Inertní gel (silikon, PE) – rozhraní sérum-krvinky
        = ;            &n= bsp; 
•
•
<= span lang=3DCS style=3D'mso-special-format:lastCR;display:none'>
gel
sérum
po centrifugaci p= 45;i 1000-1500g 10 min   = ;
         20-25 °C, stabilita 2-3 dny=
ρ =3D 1,04 g/ml
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 Plasma versus Sérum<= span style=3D'font-family:Arial;mso-ascii-font-family:Arial;mso-hansi-font-fami= ly: Arial;font-size:183%;color:yellow;mso-special-format:lastCR;display:none'>=
sérum obsahuje mnohonásobně nižší zastoupení <= /span>koagulační= ch faktorů (Ca2+, fibrinogen,...) a Mg2+
•
srážení krve – obsah rozpadlých trombocytů a    
        = ;       erytrocytů =3D> ↑ ACP, K v séru
•
Po sražení krve a separaci krvinek získáme = sérum =
Při manipulaci se srážlivou krví dbát na dodržení
        = ;            &n= bsp; doby srážení =3D 30 min !!!
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Vybrané analyty v plasmě a séru
•Vyšší hodnoty v plasmě:
•    = TP (o 4%), LD (o 2,7%), vápník (0,9%), chloridy (0,2%)
•<= /span>
•Vy&= #353;ší hodnoty v séru:
•    draslík (o 8,4= %), fosfáty (7%), GLU (5,1%), CK (2,1%), uhličitany (1,8%), ALP (1,6%), Alb (1,3%), AST (0,9%), UREA (0,6%), KM (0,2%), sodík (0,1%)
!!! sorpce Ca2+, Mg2+  na fibrin – použ&iacut= e;vat pouze sérum
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Antikoagulancia=
•princip:  vazba Ca2+ ( i Mg2+) z krve, aktivace antitrombinu III (heparin)
•<= /span>
•heparinát lithný, amonný, draselný, sodný (!)
•Na2EDTA; K2EDTA; K3EDTA
•citrát sodný, draselný, amonný, ACD
•oxalát draselný, amonný (+ NaF u GLU v krvi)
•<= /span>
•pro vyšetření z plné krve (ISE elektrody, POCT)
•vyšetřen&iac= ute; v plasmě (fibrinogen, statimová vyšetř.)
•vyšetřen&iac= ute; krvinek (hematologie)
•promytí krvinek= (HbA1c), separace leukocyt&#= 367;
•analýza<= span lang=3DCS style=3D'font-family:Arial;mso-ascii-font-family:Arial;mso-hansi= -font-family: Arial;font-size:117%;color:#FF9966'> DNA
•
T
Y
P
Y
P
R
O
Č
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Odběr venózní krve
<= span style=3D'font-family:Arial;color:white;mso-color-index:0'>•<= /span>odběr vsedě až pololeže&#= 13;
<= span style=3D'font-family:Arial;color:white;mso-color-index:0'>•<= /span>! vstoje hemokoncentrace, ↑ krev. bílkovin
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•Desinfekce kůže (= alkohol, hemolýz= a)
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•Stažení paže <= span lang=3DCS style=3D'font-family:Arial;mso-ascii-font-family:Arial;mso-bidi-= font-family: Arial;mso-hansi-font-family:Arial;color:white;mso-color-index:0'>< 1 min (hemokonc., ↓ pH, ↑ laktát, K, CK)
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>!! staženou rukou necvičit !!! (raději zatnout pěst)
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>!! hematomy, jizvy =
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•!! rychlé nasávání krve (hemolýza)
<= span style=3D'font-family:Arial;color:white;mso-color-index:0'>•<= /span>!! odběr z infúzní= ho vpichu (minerály, GLU, léky,  
•        = ;   pH, efekt lokálního naředění plasmy<= /span>)
<= span style=3D'font-family:Arial;color:white;mso-color-index:0;visibility:hidden= '>•<= /span>
<= span style=3D'font-family:Arial;color:white;mso-color-index:0;visibility:hidden= '>•<= /span>
<= span style=3D'color:white;mso-color-index:0;visibility:hidden'>•<= /span>
stoj vs leh: po 15 min až o 15%=
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Odběr kapilární krve= =
•<= /span>DM, oGTT, AB rovnov&aacut= e;ha
        = ;            &n= bsp;            = ;  – opakované odběry
•<= /span>novorozenci (prsty, pati&= #269;ka, lalůček)
        = ;            &n= bsp;            = ;   - šetrnější přístup
•! prochladnutí= konč. – lokální ↓= ; oběhu, hypoxie
•! hemolýza buněk při styku s desinf. l.
•! mačkán&= iacute; prstu – hemolýza, ↑ tkáň. mok
•
 &nbs= p;
•
•
magnet
 =   Kapilární krev obsahuje
  cca o 1% více K+, GLU  a
cca o 2-4% méně Na, Ca, Cl, 
      BIL a TP než krev žilní
Heparinizovaná krev<= /div>
------=_NextPart_01C87D67.A5C984D0 Content-Location: file:///C:/1FCBB1C1/preanalytika_soubory/slide0008_image021.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhXQEUAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABc ARMAhAAAAAAAAAAZEwANCgAKCAAPCgALCA8XFRMcGRMbGRQdGgAUDgAHBQApHwAkGgBAMABaQwB/ XwBmTACyhgDMmVE/PEhOS1JHRFReW/8AAP9AQP/r6////wECAwECAwECAwX/INOMZGk2AqCubOu+ cCzPdG3feK7vfO//wBnBcSqOHIQGZclsOimRQnBKrVqv2Kx2ezNMnuDlxKAMgyPctHrNbrvfK6/5 OS7Pm1G4fs/v+9Vyd0x1gngWG4gAiBuKiYuNjI+SjpSRlZCYk5abmZeanZyfop6koaWgqKOmq6mn qq2sr7KutKYHX4UUhLlQFxq/wMHCw8TFxsfIycrLzM3Oz9DR0tPU1ccIuIW7uREVGd/g4eLj5OXm 5+jp6uvs7e7v8PHy8/T15wnZgtuF3fb+/wADChxIsKBBdvh47RPU76DDhxAjSpxIMUPCXAvvNKzI saPHjyAPXtRGhhcUbyFTlapcybLlSH0leW1sSbOmzZv/Xt7JOGcmzp9Agwq1mG9nTG4YOChdyrSp 06dQo0qdSrWq1atYs2rdyrWr169gpSooOoenGTR/0qpdy7YtgEAk7fCT4rau3bt4gcCFKZdh3r+A Awt+S9bMmAUSEitezFjCA7qDI0ue/MYAhMaYE0MwACBA58+eQ4P+TLm06dNVRKserToEADs= ------=_NextPart_01C87D67.A5C984D0 Content-Location: file:///C:/1FCBB1C1/preanalytika_soubory/slide0008_image022.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh6wAmAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADp ACQAgwAAAAAAAAAAEgAAFAAADgAAUAAAfwAAeAAAnwAAvwAA////AAECAwECAwECAwECAwT/EMhJ q7046w2C+WAojmRpnmgqFgLnvnAsz3Q9BUqu73zv/8CgcMhD2I7IpHJ5wRGf0KjUh2gxr9istjPt er/FrXhMfjnB6PSwWm67y2e1fJ4zvu+XBZ4Wp/vBbHtZehgLhoIzfX+LUnaIWIaEE5GUlZaXmJma mRSKjJ9rVgCbpKWmp5qTo5SjrXqvrrGws7K1tLcVnqC7VBW4v7bBwMPCrqySEpGPMLq8znWiy0nK hdJmz9g9jtbT3EjN2aCB3uSI4OGM2+Xrb+fof+Ps8mTu73Tq8/lZ9fZy8foAl/DrlwZfwIM2BhIE FA2hw0QLeRl8SJGDwohd/lXcmCFAgo8gc0OKHEmypMmTKFOKPMCx5QYBA2LKnEmzps2bOHPq3FmT gMufQIMKHUq0qNGjSJMqXcq0qdOnUKNKnSqwg9UAV7Ni3aq1K9evWalSDUC2rNmzaNOqXWtW7FS2 cOPKbesW6ty7eOnWfZq37929fP0KXgtYQgQAOw== ------=_NextPart_01C87D67.A5C984D0 Content-Location: file:///C:/1FCBB1C1/preanalytika_soubory/slide0009.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Preanalytická fáze laboratorního vyšetření
Odběr arteriální krv= e=
•pro vyšetření krevních plynů, AB rovnováhy a amoniaku
•arterializovaná kapilární krev <= span lang=3DCS style=3D'font-size:133%;color:white;mso-color-index:0'>(<= /span>lal= 67;ček, pata)
•heparinizované zkumavky a kapiláry
•Ca-titrovaný heparinát lithný
•anaerobní odběr (bez bublin v kapiláře)
•
•
•
Arteriální krev obsahuje<= span style=3D'mso-spacerun:yes'>  cca o 2% více Cl než = krev žilní
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Nejčastější chyby při odběru
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>záměna žilní x arteriální x kapilární krev
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>záměna krev srážlivá (sérum) x nesrážlivá (plasma)
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>nevhodný typ antikoagulancia (citrát, heparin, EDTA)
•<= /span>chybný poměr objemů krev vs antikoagulans
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>třepán&iacu= te; zkumavky (hemolýza) - 10x promíchat
•nedodržení času srážení pro přípravu séra (fibrinový gel)
•<= /span>kontaminace jinou tkání (např. u odběru kostní dřeně)
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>odběr jiného než středního proudu moči
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>chybné nebo nevhodné značení vzorků
<= span style=3D'font-family:Arial;font-size:88%;color:white;mso-color-index:0'>•<= /span>příliš málo materiálu k provedení vyšetření=
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Transport biologického vzorku
•uzavřené standardní odběrové nádobky
•transport při 20-37 ºC  a ve tmě
•↑ teplota (> 40 ºC): denaturace enzymů, ↓ GLU
•↓ teplota (< 0 ºC): hemolýza, ↑ K+
•stanovení amoni= aku – transport při 0 ºC
•světlo: ↓ bilirubinu
•<= /span>
       !! = stanovení hodnot analytů v případě srážlivé krve nejpozději do 1H po odběru    
        = ;          (↑ K+, ↓= Cl-, ↓= ; GLU)
> 2H: raději transport séra než plné krve!!!
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Stabilita elektrolytů v nesrážlivé plné = krvi pro měření ISE elektrodami&#= 13;
        = ;            &n= bsp;            = ;   25 º= C        = ;     0 ºC=
Sodík        = ;            &n= bsp;      1H     &n= bsp;            1H
Draslík        = ;            &n= bsp;    1H     &n= bsp;            1H
Vápník        = ;            &n= bsp;  15 min        = ;      2H
Chloridy        = ;            &n= bsp;  1H        = ;          1H
Lithium        = ;            &n= bsp;   1H     &n= bsp;            1H
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Hemolýza =3D erytrolýza=
in vitro: mechanicky, osmoti= cky, tepelně, chemicky
=
LD
AST
K+
ACP
K+
K+
K+
ALT
AST
AST
LD
LD
K+
Δ pH
      Interference
při měření bilirubinu
      Interference
fotometrická (hem)
C&eacu= te;vní lumen=
 ery : plasma <= /div>
&nb= sp; LD     160 : 1
 AST      40 : 1= 3;
    K+       23 : 1 = ;
 Mg2+     2,5 : 1
   Cl<= /b>-      1,5 : 1&#= 13;
  Na+        = ;    1 : 19
Ca2+         1 : 128
Na+/K+ ATPasa
Na+
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Uchov&aacu= te;vání (skladování) vzorků krve
•20-40 ºC = plná krev:
 &nbs= p; glykol&ya= cute;za v Ery → ↓<= span lang=3DCS style=3D'font-family:Arial;mso-ascii-font-family:Arial;mso-hansi= -font-family: Arial;color:white;mso-color-index:0'> ATP → ↓<= span lang=3DCS style=3D'font-family:Arial;mso-ascii-font-family:Arial;mso-hansi= -font-family: Arial;color:white;mso-color-index:0'> membránové Na,K-ATPázy
 &nbs= p; → hyperkalémie, hyponatrémie
    Leu  ↓ O2&nbs= p; ↑ CO2        = ;  Ery&nbs= p; ↓ pH  ↑ laktátu
•
=3D> krev pro většinu BCh analytů= v chladnici
=3D> kapilárn&iacut= e; krev pro ABR: na ledu a měřit do 1H
=3D> NaF: = ↓ glykolýzy na 2= 4H: pro glykémii a laktát = ;
=3D> < 1H: oddělení plasmy (séra) od krvinek, 4°C
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Změny pH při skladování
↑ laktát
↓ pCO2
↓ iCa2+
↓ iMg2+
↓Cl-
<
∆ vazebnosti: léků, MK, BIL,…= ;
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ABYAgAAAAP///wJ4jI+py+0Po5y02guu3rz7DzpZSJbmiUJjyrbuG63wTNelbOf6rvL+D8QBh8SW sIhMfo7KpnPCfEqniSj1+rRit0gt9/vzgsc5MfkMM6PXKTX7TXLD5x05/W6x4/exjD/wFwg4KFhI eGiYiLio2Mj46BgJOSlZiVgAADs= ------=_NextPart_01C87D67.A5C984D0 Content-Location: file:///C:/1FCBB1C1/preanalytika_soubory/slide0015.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Preanalytická fáze laboratorního vyšetření
 Uchovávání vzorků sér a plasmy
      4 = ºC: cca 3-5 dní
 &nbs= p; -20 ºC: měsíce až roky
•<= /span>
 &nbs= p; -80 ºC: roky
•<= /span>
    lyofilizáty: p= ro kontrolní vzorky
------=_NextPart_01C87D67.A5C984D0 Content-Location: file:///C:/1FCBB1C1/preanalytika_soubory/slide0016.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Preanalytická fáze laboratorního vyšetření
 Uchovávání vzorků moči
•Vyšetřen&iac= ute; do 1 hodiny po odběru (AMYL, TP, UREA, Na, K, Cl), poté uchovávat v chladu
•4 ºC po 3-4 dnech: precipitace minerálů
        = ;            &n= bsp;            = ;     (soli Ca, Mg, P)
•Chemická konzer= vace a stabilizace moči:
   acidifikace moči 6M HCl: při stanovení
        = ;            &n= bsp;            = ;  Ca, Mg, P (Na, K, UREA)
   alkalizace moči 5M NaOH: stanovení KM (K, Cl, UREA, KREA)
 &nbs= p; -20 ºC: týdny až měsíce<= span style=3D'mso-spacerun:yes'>      -80 ºC: roky
•<= /span>
(na hodnotu pH ≤ 2)
(na hodnotu pH ≥ 7,5)
------=_NextPart_01C87D67.A5C984D0 Content-Location: file:///C:/1FCBB1C1/preanalytika_soubory/slide0017.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Preanalytická fáze laboratorního vyšetření
 Příprava analytického vzorku
Biologický materiál
Separace séra (plasmy)
Deproteinace<= b>
Zahuštění= <= b>
Naředění<= span style=3D'font-family:Arial;mso-ascii-font-family:Arial;mso-hansi-font-fami= ly: Arial;font-size:83%;color:yellow;mso-special-format:lastCR;display:none'><= b>
Extrakce do org. fáze<= b>
Filtrace<= b>
Izolace NK<= b>
Separace buněk<= b>
Sedimentace<= b>
Ultracentifugace<= b>
Derivatizace<= b>
Dialýza<= b>
Mineralizace<= b>
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vyšetření
3Das1479tn 3D"MODULAR
Koncentrace celkového cholesterolu v krvi:   5,0 mmol/l
Modular (Roche)
Chyba měření (analytická chyba):        = ;       ± 0,2 mmol/l
Odběr, trans= port, uložení (preanalytická): ± 0,1 mm= ol/l
Noční odběr (biologický vliv):        = ;            &n= bsp; ± 0,1 mmol/l
Dávkov&aac= ute;ní léčiva (interference):        = ;          ± 0,2 mmol/l
5,0
4,0
6,0
A/D převod, zaokrouhlení výsledku:        = ;    ± 0,1 mmol/l
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3Das1479tn 3D"MODULAR
Koncentrace celkového cholesterolu v krvi:   5,0 mmol/l
Modular (Roche)
5,0
4,0
6,0
        = ;            &n= bsp;  Nejistota výsledku měření (Uc)
        = ;            &n= bsp;      ± 0,7 mmol/l <= /b>
Interval pravděpodobného výskytu skutečné hodnoty s uvážením všech zdrojů chyb
 
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Analytická chyba
Preanalytická chyba<= /span>
Biologický vliv
Interference
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Zdroj nejistoty výsledku měření
Standardizace        analytick= ých podmínek
A/D převod, zaokrouhlení výsledku:        = ;    ± 0,1 mmol/l
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Tok informací o preanalytických vlivech
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ScaleHyperlinks( factor ) { for( ii=3D0; ii< g_HLinkArray.length; ii++) { coordsStr=3D""; imaObj =3D g_HLinkArray[ii]; for ( jj=3D0; jj < imaObj.coords.length-1; jj++ ) coordsStr +=3D (imaObj.coords[jj]*factor) + ","; coordsStr +=3D (imaObj.coords[jj]*factor); imaObj.areaObj.coords =3D coordsStr; } } function ParseCoords( coordsStr ) { var numArray =3D new Array(); var i =3D curPos =3D commaPos =3D 0; while ( ( commaPos =3D coordsStr.indexOf(",", curPos) ) !=3D -1 ) { numArray[i++] =3D parseInt( coordsStr.substr( curPos, commaPos ) ); curPos =3D commaPos + 1; } numArray[i] =3D parseInt( coordsStr.substr( curPos ) ); return numArray; } function DocumentOnClick() { if( IsNts() || parent.HideMenu() ) return; if( ( g_allowAdvOnClick && (window.name=3D=3D"PPTSld") && !parent.IsFrames= Mode() ) || (event && event.keyCode=3D=3D32) ) { if( g_animUseRuntime && g_animMainSequence && g_animMainSequence.cangonex= t ) return; parent.GoToNextSld(); } } var g_supportsPPTHTML =3D SupportsPPTHTML(), g_scaleInFrame =3D 1, gId=3D""= , g_bgSound=3D"", g_scaleHyperlinks =3D false, g_allowAdvOnClick =3D 1, g_showInBrowser = =3D 0, gLoopCont =3D 0, gUseSldTimings =3D 1; var g_showAnimation =3D g_supportsPPTHTML && SupportsPPTAnimation() && ( (w= indow.name=3D=3D"PPTSld" && !parent.IsFramesMode()) || g_showInBrowser );va= r g_animManager =3D null; var g_animUseRuntime =3D false; var g_animItemsToHide, g_animInteractiveItems, g_animSlideTime; var g_animMainSequence =3D null; var ENDSHOW_MESG=3D"Konec prezentace, klepnutím ukončíte= předvádění.", SCREEN_MODE=3D"Frames", gIsEndShow= =3D0, NUM_VIS_SLDS=3D31, SCRIPT_HREF=3D"script.js", FULLSCR_HREF=3D"fullscr= een.htm"; var gCurSld =3D gPrevSld =3D 1, g_offset =3D 0, gNtsOpen =3D gHasNts =3D gO= tlTxtExp =3D 0, gHasNarration =3D 0, gOtlOpen =3D true window.gPPTHTML=3DSupportsPPTHTML() var gMainDoc=3Dnew Array(new hrefList("slide0001.htm",1,-1,1),new hrefList(= "slide0022.htm",1,-1,1),new hrefList("slide0002.htm",1,-1,1),new hrefList("= slide0003.htm",1,-1,1),new hrefList("slide0019.htm",1,-1,1),new hrefList("s= lide0004.htm",1,-1,1),new hrefList("slide0005.htm",1,-1,1),new hrefList("sl= ide0021.htm",1,-1,1),new hrefList("slide0041.htm",1,-1,1),new hrefList("sli= de0010.htm",1,-1,1),new hrefList("slide0012.htm",1,-1,1),new hrefList("slid= e0038.htm",1,-1,1),new hrefList("slide0011.htm",1,-1,1),new hrefList("slide= 0006.htm",1,-1,1),new hrefList("slide0008.htm",1,-1,1),new hrefList("slide0= 009.htm",1,-1,1),new hrefList("slide0058.htm",1,-1,1),new hrefList("slide00= 13.htm",1,-1,1),new hrefList("slide0040.htm",1,-1,1),new hrefList("slide001= 8.htm",1,-1,1),new hrefList("slide0014.htm",1,-1,1),new hrefList("slide0039= .htm",1,-1,1),new hrefList("slide0015.htm",1,-1,1),new hrefList("slide0016.= htm",1,-1,1),new hrefList("slide0017.htm",1,-1,1),new hrefList("slide0020.h= tm",1,-1,1),new hrefList("slide0033.htm",1,-1,1),new hrefList("slide0034.ht= m",1,-1,1),new hrefList("slide0035.htm",1,-1,1),new hrefList("slide0050.htm= ",1,-1,1),new hrefList("slide0059.htm",1,-1,1)); function FullScrInit() { g_allowAdvOnClick =3D GetCurSld().mAdvOnClk document.body.style.backgroundColor=3D"black" document.oncontextmenu=3Dparent._CM; document.onkeydown =3D _KDH; document.ondragstart=3DCancel document.onselectstart=3DCancel self.focus() } function Redirect( frmId ) { var str=3Ddocument.location.hash,idx=3Dstr.indexOf('#'), sId=3DGetSldId() if(idx>=3D0) str=3Dstr.substr(1); if( window.name !=3D frmId && ( sId !=3D str) ) { obj =3D GetObj("Main-File") window.location.href=3Dobj.href+"#"+sId return 1 } return 0 } var MHTMLPrefix =3D CalculateMHTMLPrefix(); function CalculateMHTMLPrefix() { if ( document.location.protocol =3D=3D 'mhtml:') { href=3Dnew String(document.location.href) Start=3Dhref.indexOf('!')+1 End=3Dhref.lastIndexOf('/')+1 if (End < Start) return href.substring(0, Start) else return href.substring(0, End) } return ''; } function GetTags(base,tag) { if(g_supportsPPTHTML) return base.all.tags(tag); else return base.getElementsByTagName(tag); } function UpdNtsPane(){ if(frames["PPTNts"]) PPTNts.location.replace( MHTMLP= refix+GetHrefObj( gCurSld ).mNtsHref ) } function UpdNavPane( sldIndex ){ if(gNavLoaded) PPTNav.UpdNav() } function UpdOtNavPane(){ if(gOtlNavLoaded) PPTOtlNav.UpdOtlNav() } function UpdOtlPane(){ if(gOtlLoaded) PPTOtl.UpdOtl() } function SetHasNts( fVal ) { if( gHasNts !=3D fVal ) { gHasNts=3DfVal UpdNavPane() } } function ToggleOtlText() { gOtlTxtExp=3D!gOtlTxtExp UpdOtlPane() } function ClearMedia() { if (PPTSld.pptSound) PPTSld.pptSound.loop =3D 0; } function FullScreen() { if ( PPTSld.g_animUseRuntime ) PPTSld.document.body.pause(); ClearMedia(); var href =3D ( document.location.protocol =3D=3D 'mhtml:') ? FULLSCR_HREF = : FULLSCR_HREF+"#"+GetHrefObj(gCurSld).mSldHref; if(PPTNav.event.ctrlKey) { var w =3D (window.screen.availWidth * 1.0) / 2.0 var h =3D w * (PPTSld.g_origH * 1.0) / PPTSld.g_origW win =3D window.open( MHTMLPrefix+href,null,"toolbar=3D0,resizable=3D1,top= =3D0,left=3D0," + "width=3D"+ w + ",height=3D" + h ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } else { win =3D window.open( MHTMLPrefix+href,null,"fullscreen=3Dyes" ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } } function ToggleVNarration() { rObj=3DPPTSld.document.all("NSPlay") if( rObj && !PPTSld.g_animUseRuntime ) { if( (rObj.playState =3D=3D 1)||(rObj.playState =3D=3D 0) ) rObj.Play() else if( rObj.playState =3D=3D 2 ) rObj.Pause() else return; } else if( PPTSld.g_animUseRuntime ) { narObj =3D PPTSld.document.all("narrationID") if( narObj ) narObj.togglePause() } } function GetCurSldNum() { obj=3DGetHrefObj(gCurSld) if( obj.mOrigVis =3D=3D 1 ) return obj.mSldIdx else return gCurSld } function GetNumSlds() { if( GetHrefObj(gCurSld).mOrigVis =3D=3D 1 ) return GetSldList().mNumVisSlds; else return GetSldList().mList.length } function GetSldNum( href ) { for(ii=3D0; ii 1 ) PopSldList(); else if( !IsFramesMode() ) { if( gLoopCont ) GoToFirst() else EndShow() } } function GoToPrevSld() { ii=3DgCurSld-1 if( ii > 0 ) { obj=3DGetHrefObj(ii) while ( obj && ( obj.mVis =3D=3D 0 ) && ( ii>0 ) ) obj=3DGetHrefObj(--ii) if( ii =3D=3D 0 ) ii=3D1 GoToSldNum(ii) } } function GoToFirst(){ GoToSld( GetHrefObj(1).mSldHref ) } function GoToLast() { ii=3DGetSldList().mList.length if( ii !=3D gCurSld ) GoToSld( GetHrefObj(ii).mSldHref ) } function GoToSldNum( num ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue obj =3D GetHrefObj( num ) obj.mVis=3D1 gPrevSld=3DgCurSld gCurSld =3D num; PPTSld.location.replace(MHTMLPrefix+obj.mSldHref) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function GoToSld( href ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue GetHrefObj( GetSldNum(href) ).mVis=3D1 PPTSld.location.replace(MHTMLPrefix+href) } function SldUpdated( id ) { if( id =3D=3D GetHrefObj(gCurSld).mSldHref ) return gPrevSld=3DgCurSld gCurSld=3DGetSldNum(id) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function PrevSldViewed(){ GoToSld( GetHrefObj(gPrevSld).mSldHref ) } function HasPrevSld() { return ( gIsEndShow || ( gCurSld !=3D 1 && GetHrefO= bj( gCurSld-1 ).mVis =3D=3D 1 )||( GetCurSldNum() > 1 ) ) } function HasNextSld() { return (GetCurSldNum() !=3D GetNumSlds()) } function CloseWindow() { if( HideMenu() ) return; var event =3D PPTSld.event; if( !IsFramesMode() && event && (event.keyCode=3D=3D27 || event.keyCode=3D= =3D32 || event.type=3D=3D"click" ) ) window.close( self ); CatchNumKeys( self, event ); } function Unload() { gIsEndShow=3D0; } function SetupEndShow() { gIsEndShow=3D1; PPTSld.document.body.scroll=3D"no"; PPTSld.document.onkeypress=3DCloseWindow; PPTSld.document.onclick=3DCloseWindow; PPTSld.document.oncontextmenu=3D_CM; } function EndShow() { if( IsFramesMode() ) return if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue doc=3DPPTSld.document var dir =3D doc.body.dir if( dir !=3D "rtl" ) dir =3D "ltr"; doc.open() doc.writeln('


' + ENDSHOW_MESG + '

') doc.close() } function SetSldVisited(){ GetSldList().mList[gCurSld-1].mVisited=3Dtrue } function IsSldVisited(){ return GetSldList().mList[gCurSld-1].mVisited } function hrefList( sldHref, visible, advDelay, advClk ) { this.mSldHref=3D this.mNtsHref =3D sldHref this.mOrigVis=3D this.mVis =3D visible this.mVisited=3D false this.mAdvDelay=3D advDelay this.mAdvOnClk=3D advClk } function SldList(arr,curSld,fEnd) { this.mCurSld =3D curSld; this.mList =3D new Array(); var idx =3D 1; for(ii=3D0;ii 0) { PushSldList(sldList,fEnd); gCurSld =3D 1; } else if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue } function PushSldList(arr,fEnd) { var ii =3D gSldStack.length; gSldStack[ii] =3D new SldList(arr,gCurSld,fEnd); GoToSld( gSldStack[ii].mList[0].mSldHref ); } function PopSldList() { if (gSldStack[gSldStack.length-1].fEndShow) EndShow() else { gCurSld =3D gSldStack[gSldStack.length-1].mCurSld; gSldStack[gSldStack.length-1] =3D null; gSldStack.length--; var sldList =3D gSldStack[gSldStack.length-1]; GoToSld( sldList.mList[gCurSld - 1].mSldHref ); } } var custShowList=3Dnew Array(); function ImgBtn( oId,bId,w,action ) { var t=3Dthis t.Perform =3D _IBP t.SetActive =3D _IBSetA t.SetInactive=3D _IBSetI t.SetPressed =3D _IBSetP t.SetDisabled=3D _IBSetD t.Enabled =3D _IBSetE t.ChangeIcon =3D null t.UserAction =3D action t.ChgState =3D _IBUI t.mObjId =3D oId t.mBorderId=3D bId t.mWidth =3D w t.mIsOn =3D t.mCurState =3D 0 } function _IBSetA() { if( this.mIsOn ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _IBSetI() { if( this.mIsOn ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } function _IBSetP() { if( this.mIsOn ) { obj=3Dthis.ChgState( gShadowClr,gHiliteClr,2 ) obj.style.posLeft+=3D1; obj.style.posTop+=3D1 } } function _IBSetD() { obj=3Dthis.ChgState( gFaceClr,gFaceClr,0 ) obj.style.posTop=3D0 } function _IBSetE( state ) { var t=3Dthis GetObj( t.mBorderId ).style.visibility=3D"visible" if( state !=3D t.mIsOn ) { t.mIsOn=3Dstate if( state ) t.SetInactive() else t.SetDisabled() } } function _IBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( t.ChangeIcon ) { obj=3DGetObj(t.mObjId) if( t.ChangeIcon() ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-4)*t.mWidth else obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-0)*t.mWidth } t.SetActive() } } function _IBUI( clr1,clr2,nextState ) { var t=3Dthis SetBorder( GetObj( t.mBorderId ),clr1,clr2 ) obj=3DGetObj( t.mObjId ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-nextState)*t.mWidth-obj= .style.posTop t.mCurState=3DnextState return obj } function TxtBtn( oId,oeId,action,chkState ) { var t=3Dthis t.Perform =3D _TBP t.SetActive =3D _TBSetA t.SetInactive=3D _TBSetI t.SetPressed =3D _TBSetP t.SetDisabled=3D _TBSetD t.SetEnabled =3D _TBSetE t.GetState =3D chkState t.UserAction =3D action t.ChgState =3D _TBUI t.mObjId =3D oId t.m_elementsId=3D oeId t.mIsOn =3D 1 } function _TBSetA() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gHiliteClr,gShadowClr,0,0 ) } function _TBSetI() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) } function _TBSetP() { if( this.mIsOn ) this.ChgState( gShadowClr,gHiliteClr,1,1 ) } function _TBSetD() { this.ChgState( gFaceClr,gFaceClr,0,0 ) this.mIsOn =3D 0 } function _TBSetE() { var t=3Dthis if( !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) else t.ChgState( gShadowClr,gHiliteClr,1,1 ) t.mIsOn =3D 1 } function _TBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( !t.GetState ) return if( t.GetState() ) t.SetPressed() else t.SetActive() } } function _TBUI( clr1,clr2,lOffset,tOffset ) { SetBorder( GetObj( this.mObjId ),clr1,clr2 ) Offset( GetObj( this.m_elementsId ),lOffset,tOffset ) } function Offset( obj, top, left ){ obj.style.top=3Dtop; obj.style.left=3Dle= ft } function SetBorder( obj, upperLeft, lowerRight ) { s=3Dobj.style; s.borderStyle =3D "solid" s.borderWidth =3D 1 s.borderLeftColor =3D s.borderTopColor =3D upperLeft s.borderBottomColor=3D s.borderRightColor =3D lowerRight } function GetBtnObj(){ return gBtnArr[window.event.srcElement.id] } function BtnOnOver(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetActive() } function BtnOnDown(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetPressed() } function BtnOnOut(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetInactive() } function BtnOnUp() { b=3DGetBtnObj() if( b !=3D null ) b.Perform() else Upd() } function GetNtsState(){ return parent.gNtsOpen } function GetOtlState(){ return parent.gOtlOpen } function GetOtlTxtState(){ return parent.gOtlTxtExp } function NtsBtnSetFlag( fVal ) { s=3Ddocument.all.item( this.m_flagId ).style s.display=3D"none" if( fVal ) s.display=3D"" else s.display=3D"none" } function _BSetA_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etActive() } function _BSetI_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etInactive() } function _BSetP_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etPressed() } function _BSetA_BorderImg() { b =3D gBtnArr[this.mBorderId] if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _BSetI_BorderImg() { b =3D gBtnArr[this.mBorderId] if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } var gHiliteClr=3D"THREEDHIGHLIGHT",gShadowClr=3D"THREEDSHADOW",gFaceClr=3D"= THREEDFACE" var gBtnArr =3D new Array() gBtnArr["nb_otl"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOtlPan= e,GetOtlState ) gBtnArr["nb_otlElem"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOt= lPane,GetOtlState ) gBtnArr["nb_nts"] =3D new ImgBtn( "nb_nts","nb_ntsBorder",10,parent.ToggleN= tsPane ) gBtnArr["nb_nts"].SetActive =3D _BSetA_BorderImg; gBtnArr["nb_nts"].SetInactive =3D _BSetI_BorderImg; gBtnArr["nb_ntsBorder"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.= ToggleNtsPane,GetNtsState ) gBtnArr["nb_ntsElem"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.To= ggleNtsPane,GetNtsState ) gBtnArr["nb_prevBorder"] =3D gBtnArr["nb_prev"]=3D new ImgBtn( "nb_prev","n= b_prevBorder",30,parent.GoToPrevSld ) gBtnArr["nb_nextBorder"] =3D gBtnArr["nb_next"]=3D new ImgBtn( "nb_next","n= b_nextBorder",30,parent.GoToNextSld ) gBtnArr["nb_sldshw"]=3D new ImgBtn( "nb_sldshw","nb_sldshwBorder",18,parent= .FullScreen ) gBtnArr["nb_sldshwBorder"] =3D new TxtBtn( "nb_sldshw","nb_sldshwBorder",pa= rent.FullScreen,null ) gBtnArr["nb_sldshwBorder"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwBorder"].SetInactive =3D _BSetI_Border; gBtnArr["nb_sldshwText"] =3D new TxtBtn( "nb_sldshw","nb_sldshwText",parent= .FullScreen,null ) gBtnArr["nb_sldshwText"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwText"].SetInactive =3D _BSetI_Border; gBtnArr["nb_voice"] =3D gBtnArr["nb_voiceBorder"] =3D new ImgBtn( "nb_voice= ","nb_voiceBorder",18,parent.ToggleVNarration ) gBtnArr["nb_otlTxtBorder"] =3D gBtnArr["nb_otlTxt"]=3D new ImgBtn( "nb_otlT= xt","nb_otlTxtBorder",23,parent.ToggleOtlText ) gBtnArr["nb_ntsBorder"].m_flagId=3D "nb_nts" gBtnArr["nb_ntsBorder"].SetFlag =3D NtsBtnSetFlag gBtnArr["nb_otlTxt"].ChangeIcon=3D GetOtlTxtState var sNext=3D"Další",sPrev=3D"Předchozí",sEnd=3D"Kon= ec prezentace",sFont=3D"Arial",sArrow=3D"Šipka",sFreeform=3D"Voln&yacu= te; tvar",sRect=3D"Obdélník",sOval=3D"Ovál" function ShowMenu() { BuildMenu(); var doc=3DPPTSld.document.body,x=3DPPTSld.event.clientX+doc.scrollLeft,y= =3DPPTSld.event.clientY+doc.scrollTop m =3D PPTSld.document.all.item("ctxtmenu") m.style.pixelLeft=3Dx if( (x+m.scrollWidth > doc.clientWidth)&&(x-m.scrollWidth > 0) ) m.style.pixelLeft=3Dx-m.scrollWidth m.style.pixelTop=3Dy if( (y+m.scrollHeight > doc.clientHeight)&&(y-m.scrollHeight > 0) ) m.style.pixelTop=3Dy-m.scrollHeight m.style.display=3D"" } function _CM() { if( !parent.IsFullScrMode() ) return; if(!PPTSld.event.ctrlKey) { ShowMenu() return false } else HideMenu() } function BuildMenu() { if( PPTSld.document.all.item("ctxtmenu") ) return var mObj=3DCreateItem( PPTSld.document.body ) mObj.id=3D"ctxtmenu" mObj.style.visibility=3D"hidden" var s=3DmObj.style s.position=3D"absolute" s.cursor=3D"default" s.width=3D"120px" SetCMBorder(mObj,"menu","black") var iObj=3DCreateItem( mObj ) SetCMBorder( iObj, "threedhighlight","threedshadow" ) iObj.style.padding=3D2 CreateMenuItem( iObj,sNext,M_GoNextSld,M_True ) CreateMenuItem( iObj,sPrev,M_GoPrevSld,M_HasPrevSld ) CreateSeparator( iObj ) CreateMenuItem( iObj,sEnd,M_End,M_True ) mObj.style.visibility=3D"visible" } function Cancel() { window.event.cancelBubble=3Dtrue; window.event.returnVa= lue=3Dfalse } function Highlight() { ChangeClr("activecaption","threedhighlight") } function Deselect() { ChangeClr("threedface","menutext") } function Perform() { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) e.Action() else PPTSld.event.cancelBubble=3Dtrue } function ChangeClr( bg,clr ) { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) { e.style.backgroundColor=3Dbg e.style.color=3Dclr } } function M_HasPrevSld() { return( parent.HasPrevSld() ) } function M_GoNextSld() { if( gIsEndShow ) M_End(); else GoToNextSld() } function M_GoPrevSld() { if( gIsEndShow ) { gIsEndShow=3D0; history.back();= PPTSld.event.cancelBubble=3Dtrue; } else GoToPrevSld() } function M_True() { return true } function M_End() { window.close( self ) } function CreateMenuItem( node,text,action,eval ) { var e=3DCreateItem( node ) e.type=3D"menuitem" e.Action=3Daction e.IsActive=3Deval e.innerHTML=3Dtext if( !e.IsActive() ) e.style.color=3D"threedshadow" e.onclick=3DPerform e.onmouseover=3DHighlight e.onmouseout=3DDeselect s=3De.style; s.fontFamily=3DsFont s.fontSize=3D"9pt" s.paddingLeft=3D2 } function CreateSeparator( node ) { var sObj=3DCreateItem( node ) SetCMBorder(sObj,"menu","menu") var s=3DsObj.style s.borderTopColor=3D"threedshadow" s.borderBottomColor=3D"threedhighlight" s.height=3D1 s.fontSize=3D"0px" } function CreateItem( node ) { var elem=3DPPTSld.document.createElement("DIV") node.insertBefore( elem ) return elem } function SetCMBorder( o,ltClr,rbClr ) { var s=3Do.style s.backgroundColor=3D"menu" s.borderStyle=3D"solid" s.borderWidth=3D1 s.borderColor=3DltClr+" "+rbClr+" "+rbClr+" "+ltClr } ------=_NextPart_01C87D67.A5C984D0 Content-Location: file:///C:/1FCBB1C1/preanalytika_soubory/fullscreen.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; 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charset="us-ascii" Preanalytická fáze laboratorního vyšetření ------=_NextPart_01C87D67.A5C984D0 Content-Location: file:///C:/1FCBB1C1/preanalytika_soubory/outline.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii"
 Po= známky
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